![using unstained protein ladder western blot using unstained protein ladder western blot](https://i.ebayimg.com/images/g/D4UAAOSw8w1YBP5S/s-l640.jpg)
More than half of the bands for Biorad product disappears or becomes very faint. In comparison, Thermo’s 10 KD band is almost completely disappeared, and the two red bands is very faint. After overnight washing, only 25K and 20K of our marker are weakened, and the rest of the bands are still very clear. After 3 hours, all bands of our products are very clear while the brightness of Thermo’s 10 kD band has been extremely weak and Biorad’s 25K band has completely disappeared, and its 10 KD band is also extremely weak. In a controlled experiment, our marker and ones of Thermo and Biorad are subjected to 3 hours to overnight washing with TBST washing buffer. It can take as short as 3 hours or even overnight. The membrane (PVDF or NC) after transferring during western blot experiment needs to undergo blocking, primary and secondary antibody incubation, and a multi-step washing process in between. Note that the red band in Thermo’s product cannot be scanned by NIR fluorescence either. All bands except orange ones are well compatible with near-infrared (NIR) fluorescent system. The images are produced from a single controlled experiment. Dyes only fade slightly and very little or almost no degradation of the marker proteins occurs even under the extreme temperature (37 ☌) ) for one month (lanes 2 and 4). The results for a comparative study under two different temperatures (-20☌ and 37☌) are shown in this image. All the markers are subject to strict stability QC before release. Our Prestained Protein Markers are very stable. Even more, Thermo has many inconsistencies in the molecular weight between its prestained and non-prestained protein markers. Biorad ladder has two inaccurate bands of 37K and 75K while Thermo prestained products have inconsistent 35K and 25K.
![using unstained protein ladder western blot using unstained protein ladder western blot](https://www.diagenode.com/img/applications/blue_ladder_1.jpg)
Biorad and Thermo (Compare lanes 3, 4 and 5 with ours lane 6). MW Precision NewEast Biosciences Prestained Protein Marker is as accurate as the current non-prestained ladders on the market (Compare lanes 1 and 2 with ours lane 6), but has superior MW precision in comparison with the major market suppliers, ie. Highlights – Broad MW range (10-250 kD)Ģ. Ready to use, with no heating, dilution or additional reducing agent required.ġ. The protein markers are highly stable with minimal band broadening during storage. Markers are confirmed in Tris-Glycine SDS-PAGE system with an accuracy of >95% by using unstained protein ladders. Red and green bandsĪt 25 kD and 70 kD and provide easy references for molecular weight identification. # : 22301ĭescription: Tri‐Color Prestained Protein Marker – RBGĭual-Color Prestained Protein Markers are a mixture of recombinant proteins ranging from 10 kD to 250 kD. D-2010, D-2020, and D-2030) are compatible with those staining.Tri‐Color Prestained Protein Marker – RBG Cat. Can I use this product for Coomassie Blue of Silver staining? :Īll our AccuLadder™ Protein size marker (Cat.If the bands still show low sensitivity, of if a midi-gel or a large-gel is used, try increasing the loading amount of the product. We recommend to use 5 ul of the solution per well with mini gel having an area of 10x8(cm2) and thickness of 0.75 or 1.0 mm My protein bands are not clear on SDS-PAGE Gel.You do not need to add any loading buffers or undergo heat treatment. This product can be used immediately without any additional procedures. What sholud I do beforehand before using this product? :.For accurate results, we recommend using a mass spectrometer for small proteins or peptides.D-2020) product can be used to identify between 6.5 and 66 kDa. AccuLadder™ Protein size marker (Low) (Cat. D-2010) is a unstained protein size marker that can be used to identify proteins between 6.5 and 116 kDa. AccuLadder™ Protein size marker (Broad) (Cat.D-2030) can identifiy proteins between 11 and 245 kDa. AccuLadder™ 3-color Prestained Protein size marker (Broad) (Cat.Can I use this product with protein less than 10 kDa? :.